آزمایشگاه شیمی
طرز تهیه محلولهای بافر (تامپون)
در ادامه متن طرز تهيه انواع محلولهاي بافر جهت علاقه مندان به آزمايشهاي شيمي به ويژه دانشجويان رشته شيمي آورده شده است كه براي مشاهده آنها روي ادامه متن كليك كنيد
طرز تهیه محلولهای بافر(تامپون) :
اقتباس شده از :
Electrolyte solutions, the measurement and interpretation of conductance, chemical potential, and diffusion in solutions of simple electrolytes
ویرایش دوم
تالیف :Robinson, R. A., and Stokes, R. H.rev. London, Butterworths, 1968.
ویرایش دوم
تالیف :Robinson, R. A., and Stokes, R. H.rev. London, Butterworths, 1968.
Buffer solutions
A. 50 mL 0.2 M KCl + volume indicated (in mL) 0.2 M HCl
B. 100 mL 0.1 M potassium hydrogen phthalate + volume indicated (in mL) 0.1 M HCl
C. 100 mL 0.1 M potassium hydrogen phthalate + volume indicated (in mL) 0.1 M NaOH
D. 100 mL 0.1 M KH2PO4 + volume indicated (in mL) 0.1 M NaOH
E. 100 mL 0.1 M tris (hydroxymethyl) aminomethane + volume indicated (in mL) 0.1 M HCl
F. 100 mL 0.025 M Na2B4O7.10H2O (borax) + volume indicated (in mL) 0.1 M HCl
G. 100 mL 0.025 M Na2B4O7.10H2O (borax) + volume indicated (in mL) 0.1 M NaOH
H. 100 mL 0.05 M NaHCO3 + volume indicated (in mL) 0.1 M NaOH
I. 100 mL 0.05 M Na2HPO4 + volume indicated (in mL) 0.1 M NaOH
J. 50 mL 0.2 M KCl + volume indicated (in mL) 0.2 M NaOH
B. 100 mL 0.1 M potassium hydrogen phthalate + volume indicated (in mL) 0.1 M HCl
C. 100 mL 0.1 M potassium hydrogen phthalate + volume indicated (in mL) 0.1 M NaOH
D. 100 mL 0.1 M KH2PO4 + volume indicated (in mL) 0.1 M NaOH
E. 100 mL 0.1 M tris (hydroxymethyl) aminomethane + volume indicated (in mL) 0.1 M HCl
F. 100 mL 0.025 M Na2B4O7.10H2O (borax) + volume indicated (in mL) 0.1 M HCl
G. 100 mL 0.025 M Na2B4O7.10H2O (borax) + volume indicated (in mL) 0.1 M NaOH
H. 100 mL 0.05 M NaHCO3 + volume indicated (in mL) 0.1 M NaOH
I. 100 mL 0.05 M Na2HPO4 + volume indicated (in mL) 0.1 M NaOH
J. 50 mL 0.2 M KCl + volume indicated (in mL) 0.2 M NaOH
|
Buffer A
|
Buffer B
|
Buffer C
|
Buffer D
|
Buffer E
|
|||||
|
pH
|
volume
added
|
pH
|
volume
added
|
pH
|
volume
added
|
pH
|
volume
added
|
pH
|
volume
added
|
|
1.00
|
134.0
|
2.20
|
99.0
|
4.10
|
2.6
|
5.80
|
7.2
|
7.00
|
93.2
|
|
1.10
|
105.6
|
2.30
|
91.6
|
4.20
|
6.0
|
5.90
|
9.2
|
7.10
|
91.4
|
|
1.20
|
85.0
|
2.40
|
84.4
|
4.30
|
9.4
|
6.00
|
11.2
|
7.20
|
89.4
|
|
1.30
|
67.2
|
2.50
|
77.6
|
4.40
|
13.2
|
6.10
|
13.6
|
7.30
|
86.8
|
|
1.40
|
53.2
|
2.60
|
70.8
|
4.50
|
17.4
|
6.20
|
16.2
|
7.40
|
84.0
|
|
1.50
|
41.4
|
2.70
|
64.2
|
4.60
|
22.2
|
6.30
|
19.4
|
7.50
|
80.6
|
|
1.60
|
32.4
|
2.80
|
57.8
|
4.70
|
27.2
|
6.40
|
23.2
|
7.60
|
77.0
|
|
1.70
|
26.0
|
2.90
|
51.4
|
4.80
|
33.0
|
6.50
|
27.8
|
7.70
|
73.2
|
|
1.80
|
20.4
|
3.00
|
44.6
|
4.90
|
38.8
|
6.60
|
32.8
|
7.80
|
69.0
|
|
1.90
|
16.2
|
3.10
|
37.6
|
5.00
|
45.2
|
6.70
|
38.6
|
7.90
|
64.0
|
|
2.00
|
13.0
|
3.20
|
31.4
|
5.10
|
51.0
|
6.80
|
44.8
|
8.00
|
58.4
|
|
2.10
|
10.2
|
3.30
|
25.8
|
5.20
|
57.6
|
6.90
|
51.8
|
8.l0
|
52.4
|
|
2.20
|
7.8
|
3.40
|
20.8
|
5.30
|
63.2
|
7.00
|
58.2
|
8.20
|
45.8
|
|
|
|
3.50
|
16.4
|
5.40
|
68.2
|
7.10
|
64.2
|
8.30
|
39.8
|
|
|
|
3.60
|
12.6
|
5.50
|
73.2
|
7.20
|
69.4
|
8.40
|
34.4
|
|
|
|
3.70
|
9.0
|
5.60
|
77.6
|
7.30
|
74.0
|
8.50
|
29.4
|
|
|
|
3.80
|
5.8
|
5.70
|
81.2
|
7.40
|
78.2
|
8.60
|
24.4
|
|
|
|
3.90
|
2.8
|
5.80
|
84.6
|
7.50
|
82.2
|
8.70
|
20.6
|
|
|
|
4.00
|
0.2
|
5.90
|
87.4
|
7.60
|
85.6
|
8.80
|
17.0
|
|
|
|
|
|
|
|
7.70
|
88.4
|
8.90
|
14.0
|
|
|
|
|
|
|
|
7.80
|
90.6
|
9.00
|
11.4
|
|
|
|
|
|
|
|
7.90
|
92.2
|
|
|
|
|
|
|
|
|
|
8.00
|
93.4
|
|
|
|
Buffer F
|
Buffer G
|
Buffer H
|
Buffer I
|
Buffer J
|
|||||
|
pH
|
volume
added
|
pH
|
volume
added
|
pH
|
volume
added
|
pH
|
volume
added
|
pH
|
volume
added
|
|
8.00
|
41.0
|
9.20
|
1.8
|
9.60
|
10.0
|
10.90
|
6.6
|
12.00
|
12.0
|
|
8.10
|
39.4
|
9.30
|
7.2
|
9.70
|
12.4
|
11.00
|
8.2
|
12.10
|
16.0
|
|
8.20
|
37.6
|
9.40
|
12.4
|
9.80
|
15.2
|
11.10
|
10.2
|
12.20
|
20.4
|
|
8.30
|
35.4
|
9.50
|
17.6
|
9.90
|
18.2
|
11.20
|
12.6
|
12.30
|
25.6
|
|
8.40
|
33.2
|
9.60
|
22.2
|
10.00
|
21.4
|
11.30
|
15.2
|
12.40
|
32.4
|
|
8.50
|
30.4
|
9.70
|
26.2
|
10.10
|
24.4
|
11.40
|
18.2
|
12.50
|
40.8
|
|
8.60
|
27.0
|
9.80
|
30.0
|
10.20
|
27.6
|
11.50
|
22.2
|
12.60
|
51.2
|
|
8.70
|
23.2
|
9.90
|
33.4
|
10.30
|
30.4
|
11.60
|
27.0
|
12.70
|
64.4
|
|
8.80
|
19.2
|
10.00
|
36.6
|
10.40
|
33.0
|
11.70
|
32.4
|
12.80
|
82.4
|
|
8.90
|
14.2
|
10.10
|
39.0
|
10.50
|
35.6
|
11.80
|
38.8
|
12.90
|
106.0
|
|
9.00
|
9.2
|
10.20
|
41.0
|
10.60
|
38.2
|
11.90
|
46.0
|
13.00
|
132.0
|
|
9.10
|
4.0
|
10.30
|
42.6
|
10.70
|
40.4
|
12.00
|
53.8
|
|
|
|
|
|
10.40
|
44.2
|
10.80
|
42.4
|
|
|
|
|
|
|
|
10.50
|
45.4
|
10.90
|
44.0
|
|
|
|
|
|
|
|
10.60
|
46.6
|
11.00
|
45.4
|
|
|
|
|
|
|
|
10.70
|
47.6
|
|
|
|
|
|
|
|
|
|
10.80
|
48.5
|
|
|
|
|
|
|
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