Provides an introduction to how solutes can interact with columns containing porous particles and stationary phases to result in separation. The efficiency, retention and selectivity terms in the resolution equation will be explained as well as how certain particle and column characteristics can alter the terms and affect resolution.
Reviews the use of Ascentis Express columns with traditional instruments and with ultra-high pressure systems. Highlighted features include high sample throughput, low solvent consumption, column ruggedness, high sensitivity, and LC-MS compatibility.
The Use of Alternate Selectivity in Reversed-Phase HPLC (43 min).
Will describe the chemical structure of various types of bonded phases. Some possible explanations why polar RP phases have different selectivity from nonpolar, straight-chain alkyl phases will be proposed, and classes of compounds that are best separated by polar RP phases will be shown.
Introduction to Ascentis HPLC Columns (23 min).
Provides an overview of the family of columns. Ascentis columns are retentive, high performance phases that are designed specifically for separation of small molecules. Method development is made easy because all Ascentis columns have comparable retention.
Demonstrates the proper use of our exclusive fittings designed for use with ultra-high pressure and narrow-bore HPLC columns. These finger-tight fittings are rated to 15,000 psi, and are specially designed to eliminate dead volume that contributes to peak broadening and decreased resolution.